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FACT: The bloodstains were applied to the cloth prior to the formation of the image.

Working independantly with a larger sample of blood containing fibrils, pathologist Pier Baima Bollone, using immunochemistry, confirms Heller and Adler’s findings and identifies the blood of the AB blood group.

The debate on the authenticity of the shroud focuses on whether this image was transferred to the linen by some means from a real corpse or whether it was artificed by a clever forger.

Chief among the proponents of the image as a "painting" was W. Mc Crone, one of the most respected names in particle analysis.

FACT: The bloodstains on the cloth are not artist’s pigment but are real blood.

Applying pleochroism, birefringence and chemical analysis, they determined that, unlike artist’s pigment which contains iron oxide contaminated with manganese, nickel and cobalt, the iron oxide on the shroud was relatively pure.

They discovered, through research into the procedures of flax preparation and linen manufacture, that pure iron oxide is normal to the process of fermenting (retting) the flax in large outdoor vats of water.

FACT: The iron oxide, abundant on the linen of the shroud is not the remnant of artist’s pigment. Adler then proceeded to apply microspectrophotometric analysis of a "blood particle" from one of the fibrils of the shroud and unmistakeably identified hemoglobin in the acid methemoglobin form due to great age and denaturation.

Further tests by Heller and Adler established, within scientific certainty, the presence of porphyrin, bilirubin, albumin and protein.